M518E 09.7.NF.1
Stereoscopic Zoom Microscope SMZ745T
Instructions
Thank you for purchasing a Nikon product.
This instruction manual is written for users of Nikon Stereoscopic Zoom Microscope SMZ745T.
To ensure correct usage, read this manual carefully before operating the product.
- No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon.
- The contents of this manual are subject to change without notice.
- Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may remain. If you note any points that are unclear or incorrect, please contact your nearest Nikon representative.
- Some of the equipment described in this manual may not be included in the set you have purchased.
- If you intend to use any other equipment with this product, read the
manual for that equipment too.
- If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.
To ensure correct and safe operation, read this manual before using the product.
Warning/Caution Symbols Used in this Manual
Although this product is designed and manufactured to be completely safe during use, incorrect usage or failure to follow the safety instructions provided may cause personal injury or property damage. To ensure correct usage, read this manual carefully before using the product. Do not discard this manual and keep it handy for easy reference.
Safety instructions in this manual are marked with the following symbols to highlight their importance. For your safety, always follow the instructions marked with these symbols.
Symbol Description
WARNING
CAUTION
Disregarding instructions marked with this symbol may lead to serious injury or death.
Disregarding instructions marked with this symbol may lead to injury or property damage.
WARNING
- Intended use of this product
This product is intended only for microscopy. Do not use it for any other purposes.
- Do not disassemble.
Disassembling this product may result in electric shock or malfunction. Do not disassemble any part that is not indicated in this manual. If you experience problems with the product, please contact your nearest Nikon representative.
- Read the instructions thoroughly.
To ensure safety, thoroughly read this manual and the manuals for other equipment to be used with this product. In particular, be sure to follow the warnings and cautions at the beginning of the manuals.
- Check the input voltage
When using an illuminator, check that the input voltage displayed on the power supply of the illuminator matches the operating voltage. Please contact your nearest Nikon representative if the displayed voltage does not match the operating voltage. Use of an improperly matched illuminator may result in damage to the equipment.
- Cautions on the power cord
The power supply is necessary for using the halogen illuminators, which are accessories. Follow the cautions below for the power cord.
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- To prevent electric shock, turn off the power switch on the power
supply before connecting or disconnecting the power cord.
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- For the power supply TN-PSE30W A (for regions with 230 V power only), the power code is specified. Be sure to use the specified power cord for the power supply. Use of other power cords may result in malfunction, overheat, or fire.
(Refer to Power Supply TN-PSE30W A (for regions with 230 V power only) on p.22.)
WARNING
- Cautions on heat from the light source
The lamp and the lamphouse become extremely hot when the lamp is turned on. Follow the cautions below to prevent burns and fire.
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- To avoid burns, do not touch the lamp and the lamphouse while
the lamp is on or for approximately thirty minutes after it has been turned off.
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- To avoid the risk of fire, do not place fabric, paper, or highly
flammable volatile materials (i.e. gasoline, petroleum benzine, paint thinner, and alcohol) near the lamphouse while the lamp is on or for about thirty minutes after it has been turned off.
- Cautions on lamp replacement
- When replacing the lamp, wait approximately thirty minutes after turning off the lamp, and make sure that the lamp and the lamphouse have cooled sufficiently.
- To prevent electric shock and product damage, turn off the power switch on the power supply before replacing the lamp.
CAUTION
- Check the light source
When using an illuminator, be sure to use the specified rating only. Use of other rating may result in damage to the equipment. Refer to the instructions for the lamp rating.
- Cautions on assembling the product
- Take care to avoid pinching your fingers and hands.
- Scratches and dirt (i.e. fingerprints) on the lenses will degrade the image quality. When assembling the product, be careful not to scratch or directly touch the lenses.
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Notes on Handling the Product
- Handle with care.
This stereoscopic microscope is a precision optical instrument. Handle the product with care and avoid physical shocks. Physical shocks during transportation and operation as well as forcible operations may result in damage to the product.
- Installation location
Note the following conditions when installing the product.
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- Install the product in a location with a temperature of 0 to +40ºC and a relative humidity of 85% or less (no condensation).
If installed in a hot or humid location, mold may form on the lenses or condensation may occur inside, resulting in poor performance or damage to the product.
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- Do not use the product in a location subject to direct sunlight.
- Install the product in a location with little vibration.
- Install the product in a location with little dust and dirt.
- Cleaning the lenses
Do not allow dust, fingerprints, or any other dirt to get on the lenses. Dirt on the lenses will degrade the image quality. If a lense becomes dirty, clean them as described below.
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- Use an air blower to blow dust away. Remove any dust by brushing
off with a soft brush or by wiping gently with a gauze, if necessary.
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- Only when there are fingerprints or grease on the lense, wipe gently with a soft, clean cotton cloth, lens tissue, or gauze dampened with a small amount of absolute alcohol (ethyl or methyl). Do not use the same part of the cloth more than once.
- Absolute alcohol is highly flammable. Handle with care. Do not use near an open flame.
- When using absolute alcohol, follow the instructions provided by
the manufacturer.
- Cleaning painted or plastic parts
Use of silicon cloth is recommended for cleaning painted parts, plastic parts and printed parts. If such a part becomes excessively dirty, wipe it gently with a gauze dampened in a mild detergent solution. Do not use organic solvents (such as alcohol, ether, or paint thinner). As this may result in deformation of the part or removal of lettering.
- Storage
- Store the product in a dry location where mold is unlikely to grow.
- Do not store the product in a location subject to direct sunlight or high temperature and humidity.
- Put a dust-proof cover over the product to protect it from dust.
- Regular inspections (Paid service)
Regular inspections are recommended in order to maintain the peak performance. Please consult your nearest Nikon representative for details about regular inspections.
The illustration shows the combination of SMZ745T zooming body, C-W10X eyepieces and C-PS plain focusing stand.
C mount fixing screw
The direction of DS camera head can be adjusted by loosening the fixing screw and rotating the C mount.
Vertical tube Eyepiece
10X, 15X, 20X and 30X
eyepieces are available.
Diopter ring
Refer to “ 4. Adjust the diopter.” on p.11.
Zooming body
Outside diameter of the part to be mounted on the arm:
ø76
Zooming body clamp screw
Fixes the zooming body to the arm.
Arm
Groove for mounting accessories
Holds the fluorescent ring illuminator and the fiber optic ring illuminator etc.
Outside diameter: ø60
Screw for mounting accessories
Holds auxiliary objectives etc.
Inside diameter screw:
M55 ´ 0.75
Stage plate
The black or white side can be chosen depending on the sample. The antistatic ESD stage plate is also available.
C mount cap
C mount
Nikon DS camera head can be attached.
Optical path switching lever
Changes the light distribution between the vertical tube and the right eyepiece tube. Refer to “6.
Change the optical path.” on p.12.
Sleeve
Zooming knob
Changes the magnification of the sample image.
Refer to “5. Change the zooming magnification.” on p.11.
Focus knob
Refer to “2. Focus on the sample.” on p.10.
Arm fixing screw
Holds the arm. The height of the arm can be adjusted high or low.
Vertical slider
Hole for clip
Holds the clip for retaining the sample.
C-PS Focusing stand
Various stands available.
Eyepiece inclination
45° from the horizontal
Screw hole for mounting an arm for halogen illuminator
Screw holes are located on both sides of the arm. Holds the G-EIA flexible arm or
Epi-arm.
Screw hole for mounting 6V10W halogen illuminator
Holds the G-LS 6V10W halogen illuminator.
Zooming knob clicks ON/OFF screw (inside)
Refer to “ON/OFF of the zooming knob clicks.” on p.11
Hexagonal wrench hanger
Stores the supplied hexagonal wrenches.
Hexagonal wrench (Large)
Supplied with the focusing stand for changing the height of the arm.
Hexagonal wrench (Small)
Supplied with the zooming body for switching ON/OFF the zooming knob clicks.
Pillar Ground jack
Refer to “Antistatic Feature” on p.15.
- If the microscope has not been assembled, first refer to Chapter III, “Assembly.”
Place the sample.Place the sample in the optical path on the stage plate of the stand. The black or white side can be chosen depending on the sample. Use the stage clips to hold the sample, if necessary.
Focus on the sample.Turning the left and right focus knobs to the same direction will raise or lower the arm (on which the zooming body is mounted) and focus on the sample.
The distance between the focus plane and the bottom surface of the zooming body is called “the working distance”. Since the working distance of the SMZ745T is 115 mm, the focusing will become easier if you set the zooming body at the position where its bottom surface is 115 mm apart from the sample surface. Refer to Table 1 (p.23) for changing the working distance when the auxiliary objectives are attached.
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The torque of the focus knob
If the torque of the focus knob is too light, the zooming body falls down by its own weight. Adjust the torque to an appropriate weight. (Refer to “7. Adjust the torque of the focus knob.” on p.14.)
Adjust the interpupillary distance.This adjustment is for adjusting the distance between both eyes of the observer.
Adjust the interpupillary distance so that the fields of view for each eye are merged into one. Move while holding each sleeve with both hands.
This adjustment is required for each observer since individual interpupillary distance vary.
The arm moves down by turning the focus knob towards the observer.
II
4 Adjust the diopter.
This adjustment is for adjusting the eyesight of the observer.
- Turn the diopter rings on both eyepieces to set them at the 0 position (match the 0 line with the index line).
- Turn the zooming knob to 5X. Focus on the sample using the focus knob. (Refer to “2. Focus on the sample.” on p.10.)
- Turn the zooming knob to 0.67X. Look into the left eyepiece with the left eye, and focus on the sample using the diopter ring on the left eyepiece. Then, look into the right eyepiece with your right eye and focus on the sample using the diopter ring on the right eyepiece.
- Repeat steps 2 and 3 until the image is kept focused even though the zooming magnification is changed. This adjustment ensures the sharp image throughout the zooming range.
This adjustment should be performed each time the observer is changed since individual eyesights vary.
5 Change the zooming magnification.
The magnification of the sample image will change by turning the left and right zooming knobs of the zooming body. Choose the desired magnification.
The right zooming knob from the viewing side has the indication of the zooming magnification. Total magnification can be calculated by multiplying the eyepiece magnification by the zooming magnification.
Note) When the auxiliary objective is attached, multiply its magnification as well.
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- ON/OFF of the zooming knob clicks The clicks on the ON/OFF of the zooming knob are available.
- Remove the small rubber cap from the
rear of the zooming body.
- Insert the supplied hexagonal wrench (small) into the screw hole on the rear of the zooming body.
Zooming
knob
ON
OFF
Microscopy Procedures II
- ON/OFF of the zooming knob clicks are adjustable by turning the inside screw.
- Return the small rubber cap to its original position.
6 Change the optical path.
Right eyepiece : Vertical tube
=100 : 0
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Right eyepiece : Vertical tube
=100 : 0
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The light distribution between the vertical tube and the right eyepiece tube can be switched with the optical path switching lever. For binocular observation, move the lever foward. When Nikon DS camera head is mounted to the C mount of the zooming body for taking microphotographs, move the lever backward.
Please refer to the instruction of the camera head for the details of the Nikon DS Camera Head operation.
Optical path switching lever
☞ • Refer to Table 2 (p.24) for Total Magnification and Real Field of Vertical Tube.
0.5X Auxiliary objective
Auxiliary adapter
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7 Image cannot be focused even with the zooming body at the highest position.For using the 0.5X auxiliary objective or for observing a tall sample, you may not be able to focus on the sample even if turning the focus knob to raise the zooming body to its highest position.
In this case, use an optional C-ER auxiliary adapter.
Note) Attach the auxiliary objective first to the zooming body, then mount the zooming body on the auxiliary adapter.
☞ • Refer to Table 3 (p.24) for Observable Sample Heights.
III
1 Place the stand on the level surface.
2 Mount the stage plate.
Press the stage plate into the stand base while pushing it against the rim in the direction shown by the arrow in the illustration.
3 The stand arm can be lowered. (For not lowering the arm, jump to step 4.)Use the hexagonal wrench (large, supplied with the focusing stand) to loosen the arm fixing screw. Reattach the arm using the screw hole at the lower side of the vertical slider. The arm will become 55 mm lower than its original position. Before tightening the screw, make sure that the two pins on the arm are inserted into the grooves in the vertical slider.
4 Mount the zooming body.
Lightly tighten the zooming body clamp screw to hold the zooming body on the arm.
Note) Do not overly tighten the zooming body clamp screw since it may cause damage to the product.
Groove
Insert the eyepieces into the eyepiece sleeves.Be sure that it is inserted all the way to the end of the sleeve.
Note) When inserting the 10X eyepiece, assure that it reaches the end of the sleeve, because the rubber cover of the 10X eyepiece will obstruct the view of the sleeve end. When inserting, hold the rubber cover instead of the diopter ring. If not, it may result in the damage to the product.
Attach the camera head (optional).Remove the C mount cap from the zooming body.
Mount the C mount adapter to DS camera head first. Then, attach the DS camera head to the C mount of the zooming body.
The direction of DS camera head can be adjusted by loosening the fixing screw and rotating the C mount.
Adjust the torque of the focus knob.Adjust the torque of the focus knob so as not to fall down the zooming body by its own weight.
Storing the tools
Increase the torque
(To reduce the torque,
turn the knob in the direction opposite to the arrow.)
The hexagonal wrenches can be stored at the rear of the focusing stand. Refer to the figure on p.9.
Assembly
III
Antistatic Feature
The SMZ745T, the C-PS/C-PSC plain focusing stand and the C-W10X eyepieces are antistatic. You can enjoy this feature when observing the sample that cannot stand much static. When using the microscope on this purpose, change the stage plate to antistatic ESD stage plate and ground the microscope through the ground jack at the rear of the plain focusing stand. The f 4 mm grounding terminal can be inserted into the ground jack.
The following accessories are also antistatic.
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- C-FPS Fluorescence Attachment
- Auxiliary Objectives (all types)
- C-FMB Focusing Mount B
- G-US1 and G-US2 Universal Table Stands
Your reticles can be attached to the eyepiece. Remove the field ring (or lens room of the 20X and 30X) from the eyepiece, then attach the reticle with its pattern surface facing down to the eyepiece, and reattach the field ring (or lens room).
☞ • Refer to Table 1 (p.23) for the size of the reticle which can be attached.
Note) The 20X and 30X eyepieces adopt the internal focusing system, and the primary image has the magnification. Use the reticle with 1.3X pattern for 20X eyepiece, and with 1.4X pattern for 30X eyepiece.
An optional reticle with cross hairs or scaled cross hairs is available for 10X eyepiece.
Reticle
Pattern surface
Field ring
Optional reticle
IV
2 Halogen Illuminators
There are two illuminators available: the G-LS of 6V10W and the C-DSLS of 6V20W.
Lamphouse Lamp socket Plug
Filter cap
ø33 mm filter attachable
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Holder and Epi arm mounting screw hole
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1 Lamp
WARNING
- When replacing the lamp, wait approximately thirty minutes after turning off the lamp, and make sure that the lamp and the lamphouse have cooled sufficiently.
- To prevent electric shock and product damage, turn off the power switch on the power supply before replacing the lamp.
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Pull the lamp socket from the lamphouse and insert the lamp straight into the socket to the end. Use halogen lamps of the specified rating only.
Then, attach the lamp socket to the lamphouse.
G-LS
C-DSLS
(GE 787 6V-10W)
Confirmation window
Lamp
(KLS JCR/M 6V20W-2)
Light shielding tube
Socket
Holder mounting screw hole
Small protrusion
Note) Do not touch the glass part of the lamp with bare hands as it may burn and stick to your skin, possibly reducing its level of light output or causing damage to the lamp. When replacing a lamp, handle it with a cloth or through its packaging until the replacement is completed. If you get fingerprints on the lamp, wipe gently with a clean cloth dampened with alcohol.
Align the protrusion on the lamphouse with the inside of the holder of the flexible arm, then insert the mounting screw from the outside of the holder and secure it.
Secure the other end of flexible arm to the left or right screw hole (for mounting an arm for halogen illuminator) on the arm of the stand by the mounting screw supplied with the flexible arm.
G-LS
Protru- sion
Flexible arm
Holder Mounting screw
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Flexible arm
Lighting area adjustment (for G-LS only)You can adjust the lighting area by moving the lamp socket back and forth. Adjust the lighting area to allow easy viewing.
Attaching filters (for C-DSLS only)Rotate and remove the filter cap at the end of the lamphouse and insert a filter. Use filters that have a diameter of 33 mm and a width of
1.5 to 2 mm.
Using Accessories IV
6 Power supplies
Use the Power Supply XN A for regions using 100 to 120 V power, and the Power Supply TN-PSE30W A for regions using 230 V power. Insert the plug of the halogen illuminator into the output connector located on the rear of the power supply.
Power Supply XN A (for regions with 100 to 120 V power only)
Input voltage display
Check that the voltage displayed matches the voltage used in your area. If the voltages do not match, do not use the power supply and contact your nearest Nikon representative.
Power cord
Fuse
Always use the specified fuse (250 V, 1 A).
(Rear view)
Power switch
Brightness adjuster
Changes the illumination brightness when rotated.
Output connector
Connect the lamphouse cable here.
Input rating |
Power supply for 100 V regions only:
100 VAC, 50/60 Hz, 30 W
Power supply for 120 V regions only:
120 VAC, 50/60 Hz, 30 W |
Output rating |
3 to 6 VAC, 3.3 A |
Fuse rating |
250 V, 1 A |
Using Accessories IV
Power Supply TN-PSE30W A (for regions with 230 V power only)
Brightness adjuster
Input voltage display
Changes the illumination brightness when rotated.
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Check that the voltage displayed matches the voltage used in your area. If the voltages do not match, do not use the power supply and contact your nearest Nikon representative.
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Output connector
Connect the lamphouse cable here.
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AC input connector
Connect the specified power cord here.
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Input rating |
230 VAC, 50/60Hz, 0.3 A |
Voltage fluctuation |
±10% |
Output rating |
6 VAC, 5 A |
Internal fuse |
Fast acting type, F1A/250 V, 5.2 ´ 20 mm |
Operating environment |
Altitude: 2000 m max.
Temperature: 0 to +40°C (indoor use only)
Relative humidity: 85% RH max.
(no condensation) Pollution level: Degree 2 Installation: Category II |
Storage environment |
Temperature: -20 to +60°C Relative humidity: 90% RH max.
(no condensation) |
Protection class |
Class I |
Power cord |
Use only the following power supply cord. Using the wrong power cord could result in danger or fire. The protection Class I equipment should be connected to PE (protective earth) terminal.
Approved according to EU/EN standards, 3 conductor grounding Type H05VV-F, 3 m long maximum, rated at 250 VAC minimum. |
Table 1: Total Magnification and Real Field of Binocular Tube
Auxiliary objective |
Working distance [mm] |
Eyepieces |
10 X
Field number 22 Reticle diameter ø25 |
15 X
Field number 16 Reticle diameter ø19 |
20 X
Field number 12.5 Reticle diameter ø19 Magnification 1.3X |
30 X
Field number 7 Reticle diameter ø12 Magnification 1.4X |
Total magnification |
Real field [mm] |
Total magnification |
Real field [mm] |
Total magnification |
Real field [mm] |
Total magnification |
Real field [mm] |
None |
115 |
6.7 to 50 X |
32.8 to 4.4 |
10 to 75 X |
23.9 to 3.2 |
13.4 to 100 X |
18.7 to 2.5 |
20.1 to 150 X |
10.4 to 1.4 |
AL0.29 X |
311 |
1.9 to 14.5 X |
113.2 to 15.2 |
3 to 21.8 X |
82.3 to 11.0 |
3.886 to 29 X |
64.3 to 8.6 |
5.8 to 43.5 X |
36.0 to 4.8 |
AL0.5 X |
211 |
3.4 to 25 X |
65.7 to 8.8 |
5 to 37.5 X |
47.8 to 6.4 |
6.7 to 50 X |
37.3 to 5.0 |
10.1 to 75 X |
20.9 to 2.8 |
AL0.7 X |
150 |
4.7 to 35 X |
46.9 to 6.3 |
7 to 52.5 X |
34.1 to 4.6 |
9.38 to 70 X |
26.7 to 3.6 |
14.1 to 105 X |
14.9 to 2.0 |
AL1.5 X |
61 |
10.1 to 75 X |
21.9 to 2.9 |
15 to 112.5 X |
15.9 to 2.1 |
20.1 to 150 X |
12.4 to 1.7 |
30.2 to 225 X |
7.0 to 0.9 |
AL2 X |
43.5 |
13.4 to 100 X |
16.4 to 2.2 |
20 to 150 X |
11.9 to 1.6 |
26.8 to 200 X |
9.3 to 1.3 |
40.2 to 300 X |
5.2 to 0.7 |
Table 2: Total Magnification and Real Field of Vertical Tube
Auxiliary objective |
Working distance
[mm] |
Vertical tube |
0.55 X Field number 11 |
Total magnification |
Real field |
[mm] |
None |
115 |
0.37 to |
2.75 |
29.9 to |
4.0 |
AL0.29 X |
311 |
0.11 to |
0.80 |
102.9 to 13.8 |
AL0.5 X |
211 |
0.18 to |
1.38 |
59.7 to |
8.0 |
AL0.7 X |
150 |
0.26 to |
1.93 |
42.6 to |
5.7 |
AL1.5 X |
61 |
0.55 to |
4.13 |
19.9 to |
2.7 |
AL2 X |
43.5 |
0.74 to |
5.50 |
14.9 to |
2.0 |
Table 3: Observable Sample Heights
(C-PS/C-PSC plain focusing stand + C-DS diascopic stand) [mm]
Auxiliary objective |
Arm normal position |
Arm lower position |
When using auxiliary adapter |
None |
0 to 91 |
0 to 36 |
42 to 147 |
AL0.29 X |
- |
- |
- |
AL0.5 X |
- |
- |
0 to 36 |
AL0.7 X |
0 to 42 |
- |
0 to 98 |
AL1.5 X |
25 to 129 |
0 to 74 |
81 to 185 |
AL2 X |
43 to 147 |
0 to 92 |
99 to 203 |