ECLIPSE

Instructions

Introduction

Thank you for purchasing a Nikon product.
This instruction manual is written for users of the Nikon ECLIPSE E200 MV Series and E200LED MV Series Microscopes.
To ensure correct usage, read this manual carefully before operating the product.

• No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon.
• The contents of this manual are subject to change without notice.
• The equipment described in this manual may differ from the actual product in its appearance.
• Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may remain. If you note any points that are unclear or incorrect, please contact your nearest Nikon representative.
• Some of the equipment described in this manual may not be included in the set you have purchased.
• If you intend to use any other equipment with this product, read the manual for that equipment too.
• If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

Introduction.................................................................................... i
Safety Precautions........................................................................ iv
WARNING and CAUTION Symbols Used in This Manual................. iv
Symbols on the Product................................................................ v
  WARNING................................................................................ vi
  CAUTION............................................................................... viii
Notes on Handling the Product...................................................... ix
Chapter 1    Nomenclature of Each Part.......................................... 2
Chapter 2    Switches and Controls................................................. 4
Chapter 3    Microscopy Procedure (Quick Reference).................... 6
Chapter 4    Microscopy Procedure (Detailed)................................ 8
Preparation Operations previous to the microscopy.......................... 8

1. Turn on the power switch................................................... 8
2. Lower the condenser slightly from its upper limit................ 8
3. Fully open the field aperture and condenser aperture diaphragms. (Field diaphragm control is only for microscopes

4. Bring the 10x objective into the optical path....................... 9
5. Place the specimen slide on the stage and bring the specimen

6. Focus on the specimen..................................................... 10
7. Adjust the diopter ring on the eyepieces........................... 12
8. Adjust the interpupillary distance..................................... 13
9. Focus on the field diaphragm and center its position.

10. Select the objective.......................................................... 15
11. Adjust the condenser aperture diaphragm........................ 15
12. Focus on the specimen..................................................... 17
13. Adjust the field diaphragm.

14. Perform the microscopy................................................... 18
15. Turn off the power switch................................................. 21

1. Oil-Immersion Observation................................................... 22
2. Torque Adjustment of the Coarse Focus Knob........................ 4
3. Upper Limit Bolt.................................................................... 24
4. Eye Level Raiser................................................................... 25
5. Photomicrography and TV Microscopy.................................... 2
6. ND Filter for the Objective..................................................... 2
7. Cord Hangers........................................................................ 2

1. Assembly of Standard Set..................................................... 28
2. Assembly of Additional Components...................................... 30
3. Replacement of Consumable Materials................................... 2

1. Combinations of 0x (Field No. 20) Eyepiece with E-Plan Objectives............................................................................ 34
2. Microscope Terminology........................................................ 34

1. Optical.................................................................................. 36
2. Operational........................................................................... 41
3. Electrical............................................................................... 4

1. Cleaning the Lenses.............................................................. 44
2. Cleaning the Microscope........................................................ 44
3. Disinfecting the Microscope................................................... 44
4. Storing the Microscope.......................................................... 4
5. Periodical Inspections (Charged)........................................... 44

1. Microscopy (Principles).......................................................... 45
2. Performance Properties......................................................... 45
3. Physical Properties................................................................ 47

Safety Precautions

Although this product is designed and manufactured to be completely safe during use, incorrect usage or failure to follow the safety instructions provided may cause personal injury or property damage. To ensure correct usage, read this manual carefully before using the product. Do not discard this manual and keep it handy for easy reference.
Safety instructions in this manual are marked with the following symbols to highlight their importance. For your safety, always follow the instructions marked with these symbols.

WARNING

1. Do not disassemble.

2. Read the instructions thoroughly.

3. Input voltage

4. Use the specified lamp. (for E200 MV series only)

• • Specified lamp

5. Cautions on lamp replacement (for E200 MV series only)
   • When replacing the lamp, wait approximately 30 minutes after turning off the lamp, and make sure that the lamp and the field lens unit have cooled sufficiently.
   • To prevent electric shock and product damage, turn off the power switch (press to the “¡” position) and unplug the power cord from the wall outlet before replacing the lamp.
   • When replacing the lamp, be sure that the lamp socket is not damaged. If it is damaged, it may cause lamp failure or overheating. In addition, install the lamp into the lamp socket until it hits the limit. If the lamp is not properly installed, it may fall off or cause overheating or smoke due to mal-connection.
   • After replacing the lamp, be sure to attach the field lens unit. Never use the product without the field lens unit attached.
   • Do not break the used lamp. It should be disposed of as an industrial waste, according to the local regulations and rules.
6. Cautions on the power cord

• • See Chapter 10, “Specifications” for the specified power cord.
  • To prevent electric shock, turn off the power switch (press to the “¡” position) before connecting or disconnecting the power cord.
  • Note that this product is classified as Class I for electric shock protection. Be sure to connect it to a protective earth terminal.

WARNING

7. Cautions on heat from the light source (for E200 MV series only)

7. • When replacing the lamp, make sure that the lamp is cool enough to touch (the light should be off at least 30 minutes).
   • Do not touch the lamp while it is on or until the lamp has been off for 30 minutes, as doing so may result in burns.
   • Never bring cloth, paper or flammable volatile substances such as gasoline, petroleum benzine, acetone, thinner, or alcohol near a hot lamp, as a fire may result.
   • Do not block the air vents on the field lens unit. If the field lens unit is covered or items are placed on the field lens unit, heat dissipation may be hindered, causing the field lens unit to become abnormally hot.
8. Notes on handling flammable solvents

• • Immersion oil (Nikon Immersion Oil for oil immersion objectives)
  • Absolute alcohol (ethyl alcohol or methyl alcohol for cleaning optical parts)
  • Petroleum benzine (for wiping off the immersion oil)
  • Medical alcohol (for disinfecting the microscope)

8. • Keep solvents away from the lamp, the field lens unit, and any other parts that may become hot.
   • Keep solvents away from the product and its surroundings when turning on/off the power switch or plugging/unplugging the power cord.
   • Be careful not to spill the solvents.
9. Notes on handling hazardous specimens

CAUTION

1. Turn off the power switch before assembling the microscope, replacing the lamp (for E200 MV series only), and plugging in or unplugging the power cord.

2. Do not wet the product or allow intrusion of foreign matters.

3. Cautions on moving the product
   • When carrying the microscope, hold the microscope firmly by the bottom front recess and the top rear.
   • When moving the product, do not hold by the focusing knobs, eyepiece tube, and stage, etc. The parts may become detached and cause the product to fall, and may also result in malfunctions and loss of precision.

4. Cautions on assembling the product
   • Take care to avoid pinching your fingers and hands.
   • Scratches and dirt (i.e. fingerprints) on optical components such as lenses and filters will degrade the microscope image. When assembling the product, be careful not to scratch or directly touch the optical components.

5. Stage rack

6. Disposal of the microscope

Notes on Handling the Product

1. Handle with care.

2. Weak electromagnetic waves

3. Installation location and storage location

• • Install the product in a location with a temperature of 0 to +40ºC and a relative humidity of 60% or less (no condensation).

• • Install the product in a place with little dust and dirt.

• • Install the product in a place with little vibration.
  • Install and store the product on a level and sturdy table or stage that can bear the weight of the product.

• • Install the product so that the power cord to the microscope can be unplugged immediately from the AC inlet in case of an emergency.
  • Avoid placing the product in direct sunlight or immediately under the room lights.

3. • Install the product at least 10 cm away from the surrounding walls.
   • Do not install the product in a closed space such as a locker or a cabinet.
   • Do not place items on the product.
4. Notes on handling the lamp (for E200 MV series only)

5. Notes on handling optical parts

6. Make sure not to hit the field lens.

   Refocusing

7. Focus knobs

8. Oil-immersion observation

9. Shipping clamps

  1 Nomenclature of Each Part

1. Main Body

2. Eyepieces

3. Eyepiece Tube

4. Objectives

5. Condenser

6. Field Lens Unit

7. Lamp (for E200 MV series only)

8. Power Cord

Chapter 1 Nomenclature of Each Part

4. Objectives

1. 7. Lamp

• • • The main body in this figure is an example of ECLIPSE E200 MV RS.

• • • The light source for E200 MV series is halogen lamp, and the light source for E200LED MV series is white LED.

  2 Switches and Controls

1. Diopter ring

2. Revolving nosepiece

3. Stage

4. Specimen holder

5. Condenser aperture diaphragm lever

6. Condenser clamp screw

7. Condenser auxiliary lens Screw on to the bottom of the condenser.
8. Filter holder

9. Longitudinal stage motion (Y Axis) knob
10. Lateral stage motion (X Axis) knob

11. Field diaphragm ring

12. Field diaphragm centering screws

13. Fine focus knob

14. Power switch

15. Brightness control dial

16. Condenser focus knob

17. Fine focus knob

18. Coarse focus Knob Used for coarse focusing. The coarse focus knob is located on the opposite side of the stage motion knobs.
19. Coarse focus knob torque adjustment ring

20. Nameplate

21. AC inlet

12. Field diaphragm centering screws

Microscopy Procedure

(Quick Reference)

(Detailed)

1. 1. Turn on the power switch.

Press the power switch to the “l” position and the lamp will come on.

1. 2. Condenser focus knob

      Lower the condenser slightly from its upper limit.

1. 1. 1. Field diaphragm ring

         Turn the field diaphragm ring to the left limit to fully open the diaphragm.

1. 1. 2. Turn the condenser aperture diaphragm lever to the left limit to fully open the diaphragm.

(2)

1. Place specimen slide on the stage with the coverglass facing upward.
2. Open the claw of the specimen holder with your finger at the root or the tip tilt and fix the specimen slide with the claw.

3. Rotate the stage motion (Y axis and X axis) knobs to bring the specimen into the optical path. Illumination light goes through the specimen.

6 Focus on the specimen.

1. Rotate the coarse focus knob to raise the stage to its upper limit.

• When you raise the stage using the coarse focus knob, take your eyes off the eyepieces and look at the gap between the upper surface of the specimen and the front of the objective from the side.
• Since the working distances of 10x and 4x objectives are long (P.34), these objectives do not touch the specimen even when the stage is raised to its upper limit provided that the slide and coverglasses of a standard thickness are used. (The standard thickness for slide is 1.2 mm and that for coverglass is 0.17 mm.)

2. Look into the eyepiece with your right or left eye, and adjust the brightness of the viewfield by turning the brightness control dial.

Turning the dial clockwise increases the brightness; turning the dial counterclockwise decreases it.)

(2)

3. Darkens

   Brightens

   Brightness control dial

   Looking into the eyepiece, slowly rotate the coarse focus knob to lower the stage. When the specimen image appears, stop rotating the knob.

Do NOT turn the coarse focus knob further after the stage has reached its lower limit. This operation will damage the focusing mechanism.

(3)

4. Looking into the eyepiece, lower the stage.

   Coarse focus knob

   

   (4)

   Fine focus knob

   Rotate the fine focus knob and precisely focus on the image.

1. Turn the diopter ring on the right and left eyepieces to align its bottom edge with the engraved base line. This is the standard position for the diopter adjustment.

2. Swing the 40x objective in the optical path. Rotate the coarse and then fine focus knobs to bring the specimen in focus. Refer to step (6) for focusing.

3. Switch back to the 10x (or 4x) objective.

4. While looking into the right eyepiece with your right eye, focus on the specimen by rotating the right diopter ring without using the focus knob.

5. Then focus with the left diopter ring.

   Use your left eye.

   While looking into the left eyepiece with your left eye, focus on the specimen by rotating the left diopter ring without using the focus knob.
6. Repeat steps (2) to (5) to check the specimen is in focus.

(1)



Match the bottom edge of the diopter ring with the engraved base line.

(2)

8 Adjust the interpupillary distance.

This adjustment is to match the distance between the eyepieces with the distance between your eyes.

Looking through the eyepieces, merge the right and left viewfields into one by turning the binocular part. When looking into the eyepieces, do it as if you look far, and you can merge easily one viewfield with another.

Changing the Eye Level

1. Check that the 10x or 4x objective is in the optical path. Turn the field diaphragm ring to its right limit to stop down the diaphragm.

2. While looking into the eyepieces, rotate the condenser focus knob so that the outline of the field diaphragm image may become clear.

When you rotate the condenser focus knob too much to pass the focal point, the color of the image outline looks reddish or bluish. Find the position that the image outline has no color, where the diaphragm image is in focus.

3. Rotate the field diaphragm centering screws until the field diaphragm image comes to the center of the viewfield.

4. Switch to the 40x objective. If the image outline blurs, rotate the condenser focus knob to obtain the best-focused position.

Note that the field diaphragm image focused with the 40x objective is not so clear as that focused with the 10x objective.

5. Turn the field diaphragm ring so that the field diaphragm image may become about the same size as the viewfield.
6. If the field diaphragm image is not centered, use the centering screws again.

If the field diaphragm image is not in the exact center of the viewfield, it will be
off-centered especially when stopped down. Perform the centering adjustment without fail.





(1)




Turn the field diaphragm ring to stop down the field diaphragm.
(2)

(4)(5)









(6)

10Select the objective.

Rotate the revolving nosepiece to the desired objective magnification.